Sweat protein components tested by SDS-polyacrylamide gel electrophoresis followed by immunoblotting.

SDS Polyacrylamide Gel Electrophoresis of Proteins.

Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes. The amount of SDS bound by a protein, and so the charge on the complex, is roughly proportional to its size. Common...

Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting.

Polyacrylamide gel electrophoresis of lysates of purified Rickettsia tsutsugamushi revealed as many as 30 polypeptide bands, including major bands corresponding to molecular sizes of 70, 60, 54 to 56, and 46 to 47 kilodaltons. Compared with the polypeptide composition of the rickettsiae of Gilliam, Karp, and Kato strains and a newly isolated Shimokoshi strain, the major polypeptide in the Kato ...

Unpredictable behaviour of mucins in SDS/polyacrylamide-gel electrophoresis.

Characterization of Bothrops Venoms of Argentina by Sds-polyacrylamide Gel Electrophoresis

Electrophoretic pattern of Bothrops alternatus, B.neuwiedi, B. ammodytoides, B. jararaca and B. jararacussu of Argentina by SDS-Polyacrylamide gel electrophoresis was carried out. Venoms used in this study came from animals (including juvenile) which live in different geographical zones. Special care was taken to not have cross-contamination of samples with blood and tissue fluids. All the snak...

Renaturation of telomere-binding proteins after the fractionation by SDS-polyacrylamide gel electrophoresis

A simple method for identification and characterization of telomere-binding proteins is described in this article. After Sodium Dodecyl Sulphate-Polyacrylamide gel electrophoresis (SDS-PAGE), proteins are eluted, renatured and used for retardation analysis with labelled oligonucleotides corresponding to human and plant of telomeric sequences. We show here that this method is efficient to recove...